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Results Found: 4

  • Next-Generation Standard RNA-Sequencing Analysis of human monocytes stimulated by HMGB1 with or without DMOG
    Internal Dataset

    Authors
    Travis Peng
    Shin-Yi Du
    Myoungsun Son
    Betty Diamond
    Description

    We report the application of standard RNA-sequencing technology for high-throughput profiling of differential gene expression in mammalian cells. This study aims to compare differential gene expression in human monocytes in the absence or presence of HMGB1 and DMOG exposure would mimic hypoxia. Human monocytes (five million cells) from a healthy donor were divided into four groups in triplicate (control,...

    Subject
    Cell Hypoxia
    COVID-19
    Hypoxia-Inducible Factor 1, alpha Subunit
    Monocytes
    Access Rights
    Free to All
    Local Expert
    Betty Diamond
    Myoungsun Son

  • Developmental differences between neonatal and adult human erythropoiesis.
    Internal Dataset

    Authors
    Hongxia Yan
    John Hale
    Julie Jaffray
    Jie Li
    10 more author(s)...
    Description

    RNA was extracted from FACS-sorted cells at 8 distinct stages of erythropoiesis, derived from CB- and PB-CD34+ cells. cDNA libraries were prepared using the Illumina TruSeq kit and sequenced on the Illumina HiSeq 2500 (Epigenomics Core of Weill Cornell Medical College, New York). This sequencing strategy produced ~15-80 million 50bp single end reads per sample. For each distinct stage of erythropoiesis,...

    Subject
    Aging
    Erythropoiesis
    RNA-Seq
    Transcriptome
    Access Rights
    Free to All
    Local Expert
    Lionel Blanc

  • Blood pressure regulation by CD4+ lymphocytes expressing choline acetyltransferase
    Internal Dataset

    Authors
    Peder Olofsson
    Michaela Oswald
    Kevin Tracey
    Description

    Blood pressure regulation is known to be maintained by a neuro-endocrine circuit, but whether immune cells contribute to blood pressure homeostasis has not been determined. We previously showed that CD4+ T lymphocytes that express choline acetyltransferase (ChAT), which catalyzes the synthesis of the vasorelaxant acetylcholine, relay neural signals. Here we show that these CD4 +CD44hiCD62L lo T helper...

    Subject
    Blood Pressure
    CD4-Positive T-Lymphocytes
    Choline O-Acetyltransferase
    Hemostasis
    Access Rights
    Free to All
    Local Expert
    Peder Olofsson

  • RNA-seq of DRG neurons isolated from alum vs. alum+KLH immunized mice
    Internal Dataset

    Authors
    Manojkumar Gunasekaran
    Prodyot Chatterjee
    Andrew Shih
    Gavin Imperato
    18 more author(s)...
    Description

    Using a combination of rigorous molecular genetic analyses, transgenic mice, and adoptive transfer experiments, we demonstrate that DRGs do not synthesize these antigen-specific antibodies, but rather sequester primarily IgG1 subtype antibodies. As revealed by RNA-seq and targeted quantitative PCR (qPCR), dorsal root ganglion (DRG) sensory neurons harvested from either naïve or immunized mice lack...

    Subject
    Antibodies
    Ganglia, Spinal
    Inflammation
    RNA-Seq
    Sensory Receptor Cells
    Sequence Analysis, RNA
    Access Rights
    Free to All
    Local Expert
    Andrew Shih
    Sangeeta Chavan